<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal;"><font face="HelveticaNeue" class="">Dear Soft Matter Colleagues and Friends,</font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal; min-height: 14px;"><font face="HelveticaNeue" class=""><br class=""></font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal;"><font face="HelveticaNeue" class=""><font class="">On Friday 10 June 2022 at 9:30 AM at the UW Faculty of Physics (Pasteura 5, Warsaw;<b class=""> </b></font><b class="">room 1.40</b>) we are hosting the final seminar of this term, during which</font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal; min-height: 14px;"><font face="HelveticaNeue" class=""><br class=""></font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal;"><font face="HelveticaNeue" class=""><b class="">Bartosz Różycki </b>(IF PAN)</font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal; min-height: 14px;"><font face="HelveticaNeue" class=""><br class=""></font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal;"><font face="HelveticaNeue" class="">will give a talk</font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal; min-height: 14px;"><font face="HelveticaNeue" class=""><br class=""></font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal;"><span class="" style="font-family: Helvetica;"><b class="">Conformational ensemble of the full-length nucleocapsid protein of SARS-CoV-2: coarse-grained simulations and SAXS experiments</b></span></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal;"><br class=""></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal;"><b class="">Abstract</b></div><div class="" style="margin: 0px; font-stretch: normal; line-height: normal;"><font face="Helvetica" class="">The nucleocapsid protein of the SARS-CoV-2 virus comprises two RNA-binding domains and three regions that are intrinsically disordered. While the structures of the RNA-binding domains have been solved using protein crystallography and NMR, current knowledge of the conformations of the full-length nucleocapsid protein is rather limited. To fill in this knowledge gap, we combined coarse-grained molecular simulations with data from small-angle X-ray scattering (SAXS) experiments using the ensemble refinement of SAXS (EROS) method. Our results show that the dimer of the full-length nucleocapsid protein exhibits large conformational fluctuations with its radius of gyration ranging from about 4 to 8 nm. The RNA-binding domains do not make direct contacts. The disordered region that links these two domains comprises a hydrophobic alpha-helix which makes frequent and nonspecific contacts with the RNA-binding domains. Each of the intrinsically disordered regions adopts conformations that are locally compact, yet on average, much more extended than Gaussian chains of equivalent lengths. We offer a detailed picture of the conformational ensemble of the nucleocapsid protein dimer under near-physiological conditions, which will be important for understanding the nucleocapsid assembly process.</font></div><div class="" style="margin: 0px; font-stretch: normal; line-height: normal;"><font face="Helvetica" class=""><br class=""></font></div><div class="" style="margin: 0px; font-stretch: normal; line-height: normal;"><img apple-inline="yes" id="6CFD398E-DA70-46A0-A3E6-69DFC636AD0A" width="451" height="212" src="cid:90A74F9A-FADE-4A77-A11C-20C529DE80F1" class=""></div><div class="" style="margin: 0px; font-stretch: normal; line-height: normal;"><font face="Helvetica" class=""><br class=""></font></div><div class="" style="font-family: HelveticaNeue; margin: 0px; font-stretch: normal; line-height: normal;">We warmly welcome everyone to attend the talk and the Soft Matter Coffee Break after the seminar, held in room 4.51 (4th floor).</div><div class="" style="font-family: HelveticaNeue;"><br class=""></div><div class="" style="font-family: HelveticaNeue;"><font face="HelveticaNeue" class=""><div class="" style="margin: 0px; font-stretch: normal; line-height: normal;"><font face="HelveticaNeue" class="">Maria Ekiel-Jeżewska</font></div><div class="" style="margin: 0px; font-stretch: normal; line-height: normal;"><font face="HelveticaNeue" class="">Maciej Lisicki</font></div><div class="" style="margin: 0px; font-stretch: normal; line-height: normal;"><font face="HelveticaNeue" class="">Piotr Szymczak</font></div><div class="" style="margin: 0px; font-stretch: normal; line-height: normal;"><font face="HelveticaNeue" class="">Panagiotis Theodorakis</font></div></font></div><br class=""><br class=""><div class=""><div dir="auto" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div dir="auto" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0); 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